| 质粒名称: | pBABEpuro-ERBB2 A775_G776insYVMA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 40982 |
| 存储实验室: | Matthew Meyerson |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE-puro |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5200 bp |
| 完整质粒大小: | 9000 bp |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ERBB2,HER2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3768 bp |
| 基因突变: | A775_G776insYVMA |
| GenBank编号 | NM_004448.2 |
| 克隆方法: | Gateway克隆 |
| 5' 测序引物 | TTTGTACACCCTAAGCCTC |
| 3' 测序引物 | GCATACTTCTGCCTGCTG |
|
文献引用方法 |
|
| 材料和方法部分: | pBABEpuro-ERBB2 A775_G776insYVMA was a gift from Matthew Meyerson (Addgene plasmid # 40982 ; http://n2t.net/addgene:40982 ; RRID:Addgene_40982) |
| 参考文献部分: | Functional analysis of receptor tyrosine kinase mutations in lung cancer identifies oncogenic extracellular domain mutations of ERBB2. Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, Meyerson M. Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug 20. 10.1073/pnas.1203201109 PubMed 22908275 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABEpuro-ERBB2 A775_G776insYVMA 8919 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABEpuro-ERBB2 A775_G776insYVMA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8919)
AUTHORS Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang
X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S,
Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK,
Meyerson M
TITLE Functional analysis of receptor tyrosine kinase mutations in lung
cancer identifies oncogenic extracellular domain mutations of ERBB2.
JOURNAL Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
20.
PUBMED 22908275
REFERENCE 2 (bases 1 to 8919)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8919
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(11..28)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(262..281)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(930..1790)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1553..1572
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1791..1895)
/gene="bla"
/label=AmpR promoter
LTR 1926..2395
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 2460..2659
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 2860..3276
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 3206..3228
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 3244..3260
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
protein_bind 3304..3328
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(7106..7130)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(7167..7187)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7172..7501
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7352..7487
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7414..7433
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7511..8110
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7511..7530)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 8007..8027
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 8331..8800
/note="long terminal repeat from Moloney murine leukemia
virus"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa
901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
1921 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
1981 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
2221 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
2281 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
2341 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
2401 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
2461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
2521 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
2581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
2641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
2701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
2761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
2821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
2881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
2941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
3001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
3061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
3121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
3181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
3241 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccagt gtggtggtac
3301 atcacaagtt tgtacaaaaa agttggcatg gagctggcgg ccttgtgccg ctgggggctc
3361 ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg cacagacatg
3421 aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca cctctaccag
3481 ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa tgccagcctg
3541 tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg
3601 aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt tgaggacaac
3661 tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc tgtcacaggg
3721 gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat cttgaaagga
3781 ggggtcttga tccagcggaa cccccagctc tgctaccaag acacgatttt gtggaaggac
3841 atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg ctctcgggcc
3901 tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag ttctgaggat
3961 tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg
4021 cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa gcactctgac
4081 tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg cccagccctg
4141 gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg gtatacattc
4201 ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt gggatcctgc
4261 accctcgtct gccccctgca caaccaagag gtgacagcag aggatggaac acagcggtgt
4321 gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga gcacttgcga
4381 gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa gaagatcttt
4441 gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa cactgccccg
4501 ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg ttacctatac
4561 atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct gcaagtaatc
4621 cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct gggcatcagc
4681 tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat ccaccataac
4741 acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa cccgcaccaa
4801 gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg cctggcctgc
4861 caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg tgtcaactgc
4921 agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc
4981 agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca gccccagaat
5041 ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc ccactataag
5101 gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct ctcctacatg
5161 cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat caactgcacc
5221 cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc cagccctctg
5281 acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt
5341 gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg gagactgctg
5401 caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa ccaggcgcag
5461 atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc tggcgctttt
5521 ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat tccagtggcc
5581 atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt agacgaagca
5641 tacgtgatgg catatgtcat ggctggtgtg ggctccccat atgtctcccg ccttctgggc
5701 atctgcctga catccacggt gcagctggtg acacagctta tgccctatgg ctgcctctta
5761 gaccatgtcc gggaaaaccg cggacgcctg ggctcccagg acctgctgaa ctggtgtatg
5821 cagattgcca aggggatgag ctacctggag gatgtgcggc tcgtacacag ggacttggcc
5881 gctcggaacg tgctggtcaa gagtcccaac catgtcaaaa ttacagactt cgggctggct
5941 cggctgctgg acattgacga gacagagtac catgcagatg ggggcaaggt gcccatcaag
6001 tggatggcgc tggagtccat tctccgccgg cggttcaccc accagagtga tgtgtggagt
6061 tatggtgtga ctgtgtggga gctgatgact tttggggcca aaccttacga tgggatccca
6121 gcccgggaga tccctgacct gctggaaaag ggggagcggc tgccccagcc ccccatctgc
6181 accattgatg tctacatgat catggtcaaa tgttggatga ttgactctga atgtcggcca
6241 agattccggg agttggtgtc tgaattctcc cgcatggcca gggaccccca gcgctttgtg
6301 gtcatccaga atgaggactt gggcccagcc agtcccttgg acagcacctt ctaccgctca
6361 ctgctggagg acgatgacat gggggacctg gtggatgctg aggagtatct ggtaccccag
6421 cagggcttct tctgtccaga ccctgccccg ggcgctgggg gcatggtcca ccacaggcac
6481 cgcagctcat ctaccaggag tggcggtggg gacctgacac tagggctgga gccctctgaa
6541 gaggaggccc ccaggtctcc actggcaccc tccgaagggg ctggctccga tgtatttgat
6601 ggtgacctgg gaatgggggc agccaagggg ctgcaaagcc tccccacaca tgaccccagc
6661 cctctacagc ggtacagtga ggaccccaca gtacccctgc cctctgagac tgatggctac
6721 gttgcccccc tgacctgcag cccccagcct gaatatgtga accagccaga tgttcggccc
6781 cagccccctt cgccccgaga gggccctctg cctgctgccc gacctgctgg tgccactctg
6841 gaaaggccca agactctctc cccagggaag aatggggtcg tcaaagacgt ttttgccttt
6901 gggggtgccg tggagaaccc cgagtacttg acaccccagg gaggagctgc ccctcagccc
6961 caccctcctc ctgccttcag cccagccttc gacaacctct attactggga ccaggaccca
7021 ccagagcggg gggctccacc cagcaccttc aaagggacac ctacggcaga gaacccagag
7081 tacctgggtc tggacgtgcc agtgtgacca actttcttgt acaaagtggt gatgtaggaa
7141 ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
7201 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
7261 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
7321 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
7381 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
7441 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
7501 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
7561 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
7621 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
7681 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
7741 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
7801 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
7861 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
7921 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
7981 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
8041 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
8101 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
8161 tgcatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
8221 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
8281 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
8341 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
8401 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
8461 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
8521 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
8581 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
8641 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
8701 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
8761 tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat
8821 taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg
8881 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgct
//
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